Box plots of MLM and MARS knockouts. Box plots representing the predicted log2 fold change (WT/KO) in gene expression after knocking out (setting mark amplitude to zero) a single mark while holding all other amplitudes at their experimental values in both the multilinear (A) and MARS (B) models. Negative shifts indicate repressive marks and positive shifts indicate activating marks. Both models show general agreement in knockout effects. Interestingly, both models choose H4R3me2 to be among the most globally repressive marks, whereas previous studies comparing H4R3me2 levels to gene expression have shown little to no correlation, suggesting the repressive character of H4R3me2 becomes apparent in a multivariate analysis of multiple modifications.