Figure 3
From: Confronting two-pair primer design for enzyme-free SNP genotyping based on a genetic algorithm
Crossover flowchart for CTPP primer design. Two P v from the population are randomly selected for crossover. At first, a six bit binary mask is generated and indicates which variables need to be exchanged. All exchanged variables are checked for violation of a constraint. If a constriction is violated, the exchanged variables will be restored, or else the process goes on to the next step.