Figure 5
From: Confronting two-pair primer design for enzyme-free SNP genotyping based on a genetic algorithm
Mutation flowchart for CTPP primer design. When a mutation operation is performed, a mutation point in P v will be randomly selected and a corresponding value generated. Then the variable will be checked for violation of a constriction. If a constriction is violated, a random point is reselected and the variable is regenerated; otherwise the original value is replaced by the variable and the process continues in the next step.