Table 1 The statistics of the designed CTPP primers showing how many primers satisfied the common constraints for SNPs of the SLC6A4 gene*
From: Confronting two-pair primer design for enzyme-free SNP genotyping based on a genetic algorithm
| Â | Â | Â | Â | Â | Â | Constraints | Â | Â | Â | Â |
|---|---|---|---|---|---|---|---|---|---|---|
ID | Â | primer length difference | GC% | GC clamp | T m | T m difference | product length | dimer | hairpin | specificity |
1 | Results | 649/864 | 1107/1152 | 645/1152 | 998/1152 | 204/864 | 615/864 | 2752/2880 | 990/1152 | 1117/1152 |
2 | Â | 684/864 | 1115/1152 | 653/1152 | 1103/1152 | 512/864 | 608/864 | 2760/2880 | 969/1152 | 1121/1152 |