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Table 1 The statistics of the designed CTPP primers showing how many primers satisfied the common constraints for SNPs of the SLC6A4 gene*

From: Confronting two-pair primer design for enzyme-free SNP genotyping based on a genetic algorithm

       Constraints     
ID   primer
length difference
GC% GC clamp T m T m difference product length dimer hairpin specificity
1 Results 649/864 1107/1152 645/1152 998/1152 204/864 615/864 2752/2880 990/1152 1117/1152
2   684/864 1115/1152 653/1152 1103/1152 512/864 608/864 2760/2880 969/1152 1121/1152
  1. * The results of ID 1 and ID2 are the parameter settings based on DeJong and Spears but ID2 is modified with an increased population size to 1000.