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Table 2 Two wild-type ADKs and a series of chimeric enzymes generated from these two enzymes[38].

From: A novel scoring function for discriminating hyperthermophilic and mesophilic proteins with application to predicting relative thermostability of protein mutants

Seq_ID

Comm_meso

Comm_hyp

Tm (°C)

Ranking

MJA

0

62

103

8

V36J

9

53

98

7

J160V

9

53

96

6

JVJ

37

25

89

4

VJV

20

42

82.5

5

V160J

51

11

74

2

J36V

53

9

73

3

MVO

62

0

69

1

  1. Comm_meso and comm_hyp are the counts of the identical residues in the MP sequence MVO and the HP sequence MJA, respectively. The last column is the relative stability ranked by our scoring function (from least to most stable).