Skip to main content

Table 1 Isotopic coding mass differences.

From: ICC-CLASS: isotopically-coded cleavable crosslinking analysis software suite

Coding

N

H (1.00728 Da)

D (2.01355 Da)

D-H (+1)

D-H (+2)

D-H (+3)

H12/D12

12

12.08736

24.1626

12.07524

6.03762

4.02508

H8/D8

8

8.05824

16.1084

8.05016

4.02508

2.68339

H4/D4

4

4.02912

8.0542

4.02508

2.01254

1.34169

  1. Isotopic coding is achieved by using light and heavy forms of the crosslinking reagents. Reaction products from an equimolar mixture of isotopically coded reagents will appear in the mass spectra as doublets of peaks of equal intensity, corresponding to the light and heavy forms of the reagents, separated by a specific mass difference resulting from the mass difference between the different forms of the reagent, and the charge state of the crosslinked ions (+1, +2, +3 are shown).
Back to article page

BMC Bioinformatics

ISSN: 1471-2105

Contact us