ICC-CLASS: isotopically-coded cleavable crosslinking analysis software suite

BMC Bioinformatics

Table 3 Mass additions for crosslinking reaction products.

The masses of the crosslinked products for the light form of the reagents can be calculated using following formulas:
[M₁₂+H]⁺ = [M₁+H]⁺ + [M₂+H]⁺ +Mip
[M₁OH+H]⁺ = [M₁+H]⁺ +MOH
[M₁i+H]⁺ = [M₁+H]⁺ +Mi
[M₁NH₂+H]⁺ = [M₁+H]⁺ +MNH₂
where H -- mass of proton; M₁, M₂ -- masses of free peptides; M₁₂ -- mass of inter-peptide crosslink; M₁OH -- mass of dead-end crosslink; M₁i -- mass of intra-peptide crosslink; M₁NH₂ -- mass of dead-end amide crosslink (if the reaction was quenched with ammonium salts); Mip, MOH, Mi -- mass additions for inter-peptide, dead-end, and intra-peptide crosslinks, respectively.

ISSN: 1471-2105