Figure 5

Example illustrating the improved binning specificity obtained using 'bit-score/distance' approach. An example illustrating the improved specificity of assignment using the 'bit-score/distance' ratios. In this example, the read originates from Burkholderia ambifaria (the corresponding sequences of which are present in the reference database). Based on the alignment parameters (Identities:I, Positives:P, Gaps) obtained for the best hit, the appropriate taxonomic level of assignment is restricted at the 'Species level'. Identified candidate ancestors are depicted in bold boxes. The read is assigned by DiScRIBinATE at the genus level (i.e. Burkholderia) on account of higher cumulative 'bit-score/distance' ratio. An LCA based approach would have assigned the same read at the phylum level (i.e Proteobacteria).