Assessment of genetic variation for the LINE-1 retrotransposon from next generation sequence data

BMC Bioinformatics

Table 2 Primers used for allele specific PCR

Locus/Allele	5' Oligo	3' Oligo	PCR Product Size(nt)
Chr5:21243489_L1+	ttgcctaagcttcccataca	TGAACCCGGTACCTCAGATG	539
Chr5:21243489_L1-	ttgcctaagcttcccataca	tgatccatgtcaataatgaggtc	677
TRDN_L1+	tttcatgccatctctatattccaa	TGAACCCGGTACCTCAGATG	477
TRDN_L1-	tttcatgccatctctatattccaa	aaaaacaggcctgagattgc	690
GLCCI1_L1+	tcctcatacaaacttgtgaagtgat	TGAACCCGGTACCTCAGATG	666
GLCCI1_L1-	tcctcatacaaacttgtgaagtgat	ttcaagcttcatctaatgaaagaaaa	682
PRKCA_L1+	tgtgcaaatttcaccccata	TGAACCCGGTACCTCAGATG	527
PRKCA_L1-	tgtgcaaatttcaccccata	ccatagagcagtgacccaca	622

Legend: Primers used for the allele specific PCR verification of the next generation sequence data analysis. The same right primer was used in all L1+ PCR reactions. This primer spans bases 90 to 109 of the LINE-1 transcript with accession L19092.

ISSN: 1471-2105