NeurphologyJ can quantify the neurite length reduction effect of nocodazole. (A) Bar graph showing the inverse relationship between the total neurite length and nocodazole concentration in P19 neurons. (B) Bar graph showing the concentration of nocodazole does not affect the soma count in P19 neurons unless a high dosage of nocodazole is added. Each bar consists of three independent experiments, and each experiment is derived from 12 different images. Error bars represent standard error of the mean. (C) Typical images of P19 neurons at various nocodazole concentrations. Original images are shown on the left and the analyzed images are shown on the right. Neurite tracings are shown in red and somata are shown in blue. The scale bars in the original images represent 50 μm.