Table 1 Default values of the most commonly adjusted LAVA parameters
Parameter | Default Target |
|---|---|
Outer primer length | 18-23 bp |
Middle primer length | 18-23 bp |
Loop primer length | 18-23 bp |
Inner primer length | 20-26 bp |
Outer primer Tm | 59-61°C |
Middle primer Tm | 59-61°C |
Loop primer Tm | 58-62°C |
Inner primer Tm | 62-66°C |
Maximum signature length | 320 bp |
Minimum spacing from middle to inner primers | 25 bp |
Maximum consecutive repeated bases | 5 |