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Table 1 Default values of the most commonly adjusted LAVA parameters

From: LAVA: An Open-Source Approach To Designing LAMP (Loop-Mediated Isothermal Amplification) DNA Signatures

Parameter Default Target
Outer primer length 18-23 bp
Middle primer length 18-23 bp
Loop primer length 18-23 bp
Inner primer length 20-26 bp
Outer primer Tm 59-61°C
Middle primer Tm 59-61°C
Loop primer Tm 58-62°C
Inner primer Tm 62-66°C
Maximum signature length 320 bp
Minimum spacing from middle to inner primers 25 bp
Maximum consecutive repeated bases 5