Intensity distribution of LC-orbitrap data. B. subtilis strain 168 was measured using the LC-orbitrap in the positive mode. The centroid data has 1 694 959 data points, obtained with 1945 scans over 45 minutes (data size is 29 MB). (a) % of total variance. Each dot corresponds to the percent of variance explained by each corresponding percent of the data from 0.1-100 at interval of 0.1%. Red dot corresponds to the top 1% of the data, which explained 99.7% of the total variance. (b) Intensity distribution. All data points are plotted. Nine black horizontal solid lines correspond to 90th- 98th percentile values at interval of 1%, and the red line corresponds to the 99th percentile value.