GAM is robust to sequencing depth and gene expression levels. Seven lanes of MAQC2 UHR data to perform GAM correction by adding one lane at a time. The log fold changes of estimated gene expression levels before and after GAM correction were calculated for genes with high, medium and low expression. Fractions of genes with log fold change within ± 5% of the final value were plotted. Overall the correction was robust to sequencing depth, with ~80% genes showing the fold change within ± 5% of the final estimates using only one lane. Moreover, genes with lower expression were only slightly more sensitive to sequencing depth.