Mapping evaluation of flow-space assisted local alignment. Showing the number of incorrectly aligned nucleotides (lines, left y-axis) as well as the per cent correctly aligned nucleotides (diamonds/squares). The test was performed through simulating 100,000 Titanium reads, at 100% down to 95% identity (x-axis) from Salmonella enterica subsp. enterica serovar Typhi str. CT18. The reads were finally aligned back against the genome and number of correctly aligned nucleotides was assessed.