Comparison of test statistics. Comparison of test statistics. The plots show average ROC curves for normal t-statistic, moderated t-statistic and effect size. Panel a shows the results when using data from two technical replicates, panel b is from two biological replicates with two technical replicates each. The moderated t-statistic and effect size outperform the ordinary t-statistic in both scenarios; on data with biological replicates, the moderated t-statistic performs better than the effect size.