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Table 3 ABP minimal cut sets (MCSs) with corresponding ABP gene(s) and reactions.

From: A systems approach to identifying correlated gene targets for the loss of colour pigmentation in plants

MCS N-3-D-RXN LEUCPEL-RXN D-4-R-RXN RXN-525 APIGNAR-RXN RXN-600 RXN-602 RXN-7652 PELUDP-RXN RXN1F-775 RXN-7775 N-C-S-RXN Total rxns Unaffected Genes/enz in MCS
1 0 0 0 0 1 0 0 0 0 0 0 0 1 3 CHI
2 0 0 0 0 0 0 0 0 0 0 0 1 1 3 CHS
3 1 0 0 0 0 1 0 0 0 0 0 0 2 57 F3H, DFR(cy)
4 1 0 0 0 0 0 0 1 0 0 0 0 2 9 F3H, F3'H(i)
5 0 1 0 0 0 1 0 0 0 0 0 0 2 126 ANS(pg), DFR(cy)
6 0 0 1 0 0 1 0 0 0 0 0 0 2 123 DFR(pg), DFR(cy)
7 1 0 0 0 0 0 1 0 0 0 0 0 2 60 F3H, ANS(cy)
8 0 1 0 0 0 0 1 0 0 0 0 0 2 132 ANS(pg), ANS(cy)
9 0 0 1 0 0 0 1 0 0 0 0 0 2 129 DFR(pg), ANS(cy)
10 0 0 0 0 0 1 0 0 1 0 0 0 2 129 DFR(cy), 3-UGT(pg)
11 0 0 0 0 0 0 1 0 1 0 0 0 2 135 ANS(cy), 3-UGT(pg)
12 1 0 0 0 0 0 0 0 0 1 0 0 2 63 F3H, 3-UGT(cy)
13 0 1 0 0 0 0 0 0 0 1 0 0 2 138 ANS(pg), 3-UGT(cy)
14 0 0 1 0 0 0 0 0 0 1 0 0 2 135 DFR(pg), 3-UGT(cy)
15 0 0 0 0 0 0 0 0 1 1 0 0 2 141 3-UGT(pg), 3-UGT(cy)
16 1 0 0 0 0 0 0 0 0 0 1 0 2 18 F3H, F3H(cy)
17 0 1 0 1 0 0 0 1 0 0 0 0 3 39 ANS, F3'H, F3'H
18 0 0 1 1 0 0 0 1 0 0 0 0 3 36 DFR, F3'H, F3'H
19 0 0 0 1 0 0 0 1 1 0 0 0 3 42 F3'H, F3'H, 3-UGT(pg)
20 0 1 0 1 0 0 0 0 0 0 1 0 3 48 ANS, F3'H, F3H(cy)
21 0 0 1 1 0 0 0 0 0 0 1 0 3 45 DFR, F3'H, F3H(cy)
22 0 0 0 1 0 0 0 0 1 0 1 0 3 51 F3'H(ii), 3-UGT(pg), F3H(cy)
fc 0.50 0.43 0.43 0.33 1 0.50 0.50 0.38 0.43 0.50 0.38 1    
Enzymes 1 2 3 4 5 6 7 8 9 10 11 12    
  1. Table 3 shows the set of MCSs or ABP gene candidates for genetic changes needed to eliminate anthocyanin production. Some reactions in the top row have been abbreviated and the numbers in the bottom row represent enzymes corresponding to the reactions in the top row; the details are as follows:
  2. Reactions (top row):
  3. N-3-D-RXN: NARINGENIN-3-DIOXYGENASE-RXN
  4. D-4-R-RXN: DIHYDROKAEMPFEROL-4-REDUCTASE-RXN
  5. N-C-S-RXN: NARINGENIN-CHALCONE-SYNTHASE-RXN
  6. Unaffected: Non-affected flav EMs
  7. Enzymes (bottom row):
  8. 1: F3H; 2: ANS/LDOX (pg); 3: DFR(pg); 4: F3'H(ii); 5: CHI; 6: DFR(cy); 7: ANS/LDOX (cy); 8: F3'H(i); 9: 3-UGT/UDPG (pg); 10: 3-UGT/UDPG (cy); 11: F3H(cy); 12: CHS
  9. The reactions directly forming the anthocyanin compounds (PELUDP-RXN and RXN1F-775) are identified as the objective functions for which the MCSs are determined. In the last column, parallel enzymes are listed by a single representative: ANS & LDOX corresponding to LEUCPEL-RXN as ANS(pg), ANS & LDOX corresponding to RXN-602 as ANS(cy); 3-UGT & UDPG corresponding to PELUDP-RXN as 3-UGT(pg) and 3UGT & UDPG corresponding to RXN1F-775 as 3-UGT(cy). fc - fragility coefficient. (Refer to Figure 3 legend or List of abbreviations used, for explanation of the abbreviated enzymes.)