1D-NMR spectra. MetaboLab graphical user interface showing an overlay of 329 1D-NMR spectra of extracted tissue biopsies before (large spectra) and after post-processing (insert spectra). Spectra from cancerous tissue are plotted in blue while those originating from control samples are shown in red. The NMR spectra were aligned to TMSP, baseline corrected and the water region and regions without peaks were excluded. The spectra were then scaled to the total spectral area and re-aligned with respect to the first spectrum.