Name
|
AmpliconNoise
|
SLP 1%
|
SLP 2%
|
DeNoiser
|
---|
3% OTUs
|
Good
|
Missed
|
Noise
|
Good
|
Missed
|
Noise
|
Good
|
Missed
|
Noise
|
Good
|
Missed
|
Noise
|
Divergent
|
26
|
0
|
1
|
26
|
1
|
162
|
26
|
1
|
11
|
24
|
0
|
3
|
Artificial
|
34
|
0
|
1
|
38
|
0
|
109
|
32
|
2
|
10
|
36
|
0
|
6
|
Even1
|
57
|
0
|
131
|
60
|
2
|
174
|
39
|
19
|
65
|
59
|
1
|
21
|
Even2
|
57
|
0
|
89
|
59
|
2
|
137
|
42
|
15
|
53
|
58
|
3
|
22
|
Even3
|
58
|
0
|
125
|
65
|
0
|
197
|
43
|
17
|
75
|
58
|
3
|
23
|
Uneven1
|
58
|
0
|
101
|
60
|
0
|
144
|
47
|
10
|
59
|
54
|
7
|
24
|
Uneven2
|
48
|
0
|
35
|
51
|
0
|
74
|
38
|
10
|
26
|
46
|
6
|
6
|
Uneven3
|
49
|
1
|
46
|
51
|
2
|
97
|
41
|
10
|
45
|
48
|
6
|
12
|
Titanium
| | | | | | | | | | | | |
σ
s
= 0.1
|
66
|
0
|
8
|
74
|
0
|
169
|
67
|
1
|
18
|
15
|
53
|
14
|
σ
s
= 0.04
|
67
|
0
|
21
| | | | | | | | | |
- Reads classified as chimeric by comparison with the references were removed prior to complete linkage OTU construction at 3% sequence difference with the corresponding reference sequences. Each OTU was then classified as 'Good' if it comprised reference sequences and denoised pyrosequenced reads, 'Missed' OTUs are formed only from reference sequences, and those OTUs containing only pyrosequenced reads are designated 'Noise'. The numbers of classified OTUs observed for each data set and noise removal algorithm are shown below.