Results of qPCR verification and amplicon design. Two of the regions chosen for verification are shown with log mean quantities of DNA measured on four replicates. The location of verified nucleosomes is indicated by dark green horizontal bars. A nucleosome prediction that is not supported by qPCR measurements is shown in red with an alternative position suggested by the PCR results indicated in blue. A light blue bar indicates the location of a nucleosome identified by qPCR that was not predicted by our analysis. Nucleosome scores are shown shaded in grey. Below the location of amplicons used to tile across the selected regions is shown relative to predicted nucleosomes.