pcrEfficiency: a Web tool for PCR amplification efficiency prediction

BMC Bioinformatics

Table 2 GAM overview

GAM analysis
	Estimate	Std. Error	t value	Pr (> \| t \|)
(Intercept)	1.73825	0.00153	1136	<2e-16
Approximate significance of smooth terms
	edf	Ref.df	F	p-value
s(lengthSequence, gcSequence)	17.58	18.08	2.332	0.00114
s(primersLength, gcPrimers)	27.96	28.46	22.950	< 2e-16
s(gcImbalance, primerDimers)	28.83	29.33	16.717	< 2e-16
R-sq.(adj) = 0.41 Deviance explained = 42.1% GCV score = 0.0094091 Scale est. = 0.0092293 n = 3944

GAM summary as estimated by the gam function of the mgcv R package. Model formula corresponds to: efficiency s(lengthSequence, gcSequence) + s(primersLength, gcPrimers) + s(gcImbalance, primerDimers).

ISSN: 1471-2105