Results of the ES compuations. (a) Computed DIF values for the positive (blue) and negative (red) controls of a randomly selected plate from the hepatitis C virus screen. Note that four positive and seven negative controls were spotted on this plate. DIF measures the difference between the running sums computed for each siRNA or well in the screen, describing the enrichment of cells from the corresponding well towards high or low signal intensities. The enrichment score ES is the maximum DIF value observed for a particular spot/siRNA under consideration. (b) Summarized enrichment scores ES of each siRNA in the HCV screen. Replicate enrichment scores were summarized using the median, siRNAs were plotted in the sequence as spotted on the plates. The red line shows the siRNAs sorted by increasing ES. (c, d) Distribution of the summarized enrichment scores ES of the full HCV and DENV screens, summarizing replicates using the median. The location of positive and negative control scores is indicated by red circles for the negative and by blue diamonds for the positive controls.