Fig. 7

The Y-SNP with test complex is shown at the base-level specification and at the diagrammatic level, where a SNP base is as indicated. If the SNP is its variant form (typically only one other base possibility is common), then a base-pairing will not occur at the nexus of the Y-SNP shown (with the red base becoming a ‘T’ in the variant as indicated). This allows discrimination between the annealed forms with high accuracy, while also discerning from the signals produced by the non-annealed Y-SNP, where there is no target-bound, or only non-specific molecular interactions imparting much less conformational structure as occurs with the matching (or mostly matching) annealing interaction.