Number of transcripts that could be aligned to the genome. Shown are the percentages of transcripts that could be successfully aligned to its corresponding genome with Dme-13g (a) and Spo-6.8g (c) data sets. (b) Percentage of unique and shared transcripts that could be successfully aligned to the genome using Dme-13g data set by each of SOAPdenovo-MK, trans-ABySS, Oases-MK and Trinity. (d) The percentage of unique unmapped-transcripts produced from each of assembly methods using Spo-6.8g data set. Numbers above the histogram are the number of unique unmapped-transcripts (left) and number of unique unmapped-transcripts that had BLASTX top hits (E≤10-10) to Uniprot database (right, within the brackets).