Differential expression orthologous miRNAs between D. simulans and D. pseudoobscura. The expression of mature miRNAs from 5’ (A) and 3’ (B) arms of the hairpin precursors were estimated separately. The normorlized expression levels of miRNAs (Log2 TPM) in D. pseudoobscura (y-axes) were plotted against those of their orhologs in D. simulans (x-axes). MiRNAs that differentially expressed between species or not were indicated as solid or open circles, respectively. Dsi, D. simulans; Dps, D. pseudoobscura; TPM, Tags per million mapped reads.