Assessment of the quality of bacterial genome assemblies. Measurements of the quality of assemblies produced by our algorithm on 369 bacterial genomes under three different optical map error rates. In each boxplot, we extend the whiskers beyond the upper and lower quartiles for 1.5 times the interquartile range, and omit outliers beyond the whiskers. (a) Sequence correctness of the assemblies, measuring the percent of the genome that was correctly assembled. (76, 72, and 65 outliers are not shown the high, medium, and low error bars, respectively.) Over ¾ of the genomes are assembled with greater than 98% sequence correctness, even in the high error setting. (b) Percent of edges assembled in the correct order by our algorithm on the 369 genomes, over three error rates. The percent of edges correct is generally lower than the sequence correctness percentages, but the difference is mostly due to short edges misplaced by the algorithm. (26, 33, and 37 outliers are not shown in the high, medium, and low error settings, respectively.) (c) N50 size of the final contigs produced by our algorithm, after breaking genomic segments at assembly errors, normalized by genome size. (54 outliers were omitted from the first bar, measuring assembly without a map.) (d) Number of contigs that would be produced with no optical map (and only the de Bruijn graph), and with optical maps simulated with three different levels of noise. (We omit 42, 53, 50, and 45 outliers in the no map, high error, medium error, and low error settings, respectively.) We see a substantial improvement in both the final number of contigs and the final N50 size, when given an optical map with any one of the three error rates.