Figure 7
From: ESTclean: a cleaning tool for next-gen transcriptome shotgun sequencing
Experiment workflow. Since SeqClean cannot trim a barcode, barcode-trimmed reads by ESTclean were used as input data. The cleaned reads by SeqClean and ESTclean were mapped to the reference genome using GMAP. We filtered out multiply mapped reads and non-overlapping reads by at least 40bp in the genome. Finally 1,290,547 reads were used for evaluation.