Figure 5

An xample of the HTML Quality Assessment Report Generated by the QUAliFiER Package. A) qaTasks are categorized by assay level, channel level, or tube level, depending on the grouping variables for outlier detection. Within each category, a summary of the number of FCS files failing that qaTask is visible. B) Clicking on the "+” signs expands a more detailed view of the qaTask, including summary plots and tables. The summary plots themselves are interactive through the use of SVG graphics. The consistency of redundant staining is shown as boxplots of the % of marker positive cells grouped by stain. C) Clicking on individual points in the summary plots opens more detailed plots of the cell populations for individual samples failing the qaTask. Densityplots of one of the outlier groups show two samples with inconsistent positive staining in the FITC channel.