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Table 1 The distribution of amino acids and codons at the 6 coevolving sites in the sequenced 92 amylase variants

From: Improving the thermostability of alpha-amylase by combinatorial coevolving-site saturation mutagenesis

Amino

Positions

 

Acid

G89

D95

H100

D144

T147

N197

No.

W. T.

GGC

GAT

CAT

GAT

ACT

AAT

 

Ala (A)

GCG2

    

CGC 1

3

Cys (C)

TTT 2

   

TG T3

TG T7

12

Asp (D)

CTG 1

    

G AT1

2

Glu (E)

GAG1,

  

GAG3

  

4

Phe (F)

TTT 1

     

1

Gly (G)

GGG5,GGT3

  

GGG4,GG T4

 

GGG 3

27

His (H)

 

C AT1

    

1

Ile (I)

  

AT T2

   

2

Lys (K)

     

AAG1

1

Leu (L)

CTG 1

     

1

Met (M)

  

ATG 4

  

ATG2

6

Asn (N)

AAT 1

 

A AT2

 

AA T3

 

6

Pro (P)

    

C CT3

 

3

Gln (Q)

CAG 1

 

CAG1

  

C AG1

3

Arg (R)

C GT3,C GC3

CGG 1

CGG1,CG T3

CGG 1

CG T4,CGC3

AGG1

20

Ser (S)

  

AG T1

 

T CT1

TCG5, AG T1

3

Thr (T)

ACA 1

     

1

Val (V)

   

GT T3

GTG 3

 

6

Trp (W)

   

TGG 1

  

1

Tyr (Y)

      

0

Type

11

2

6

5

6

9

 
  1. Subscript numbers represent the frequencies of the codons in the 92 sequenced variants. Underlined bold letters indicate substitution to the wild type nucleotide.
  2. No. = total number of codons different from the wild type for each amino acid. Type = type number of amino acids appeared at each site. W. T. = wild type.
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BMC Bioinformatics

ISSN: 1471-2105

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