Table 2 Comparison of statistical parameters used for selection of the consensus criteria obtained with the training dataset of 526 Gram-negative bacterial protein sequences of known localization and for validation of the consensus criteria obtained with the test dataset of 529 Gram-negative bacterial protein sequences of known localization

Training dataset

Subcellular localization predictor group

   At least one positive prediction

0.95

1.00

0.95

0.77

   At least two positive predictions

0.99

0.98

0.99

0.94

   At least three positive predictions

0.98

0.72

1.00

0.82

   All four positive predictions

0.96

0.51

1.00

0.70

Transmembrane β-barrel protein predictor group

   At least one positive prediction

0.50

0.95

0.46

0.23

   At least two positive predictions

0.86

0.77

0.87

0.46

   At least three positive predictions

0.91

0.56

0.95

0.47

   All four positive predictions

0.94

0.37

0.99

0.52

Outer membrane lipoprotein predictor group

   At least one positive prediction

0.93

0.35

0.98

0.41

   All two positive predictions

0.94

0.33

1.00

0.51

Test dataset

   At least two positive predictions for subcellular localization predictors

1.00

0.98

1.00

0.98

   At least three positive predictions for transmembrane β-barrel protein predictors

0.91

0.63

0.94

0.50

   At least one positive prediction for outer membrane protein predictors

0.93

0.44

0.98

0.49

   Integration of the three predictor groups

0.92

1.00

0.92

0.69