Figure 8

Verification of poor coherence. Peptide that doesn’t follow the pattern of it’s dominant metaprotein. Panel a shows the correlation between the measured intensity of the polypeptide TTPPTTATPIR from the label-free, unbiased platform compared to the label-free SRM platform. The high level of correlation demonstrates the reproducibility of measurement of this peptide. Panel b shows the pattern of expression, as measured in the unbiased platform, as compared to the pattern of expression of the FINC metaprotein. Panel c shows a scatterplot comparing the pattern of expression of the FINC metaprotein and the intensity of the peptide as measured with the shotgun approach and panel d shows the same comparison, but now with the SRM approach instead of the shotgun approach. The peptideis measured consistently and it does not correlate with the expression pattern of the FINC metaprotein.