Comparison with Solexa method. (Colored figure) Base errors per cycle for the entire dataset based on the BM-BC (top panel) and the Bustard under Solexa sequencing (bottom panel). The plot further confirms that for the BM-BC method, there is no increase in base substitution errors with increasing cycle, a common problem in most basecalling methods. Also the major potential substitution errors, A-C and G-T substitutions have been accounted for quite well. For the Bustard method, there is a large increase in the error rates (after cycle 26, shown by the green dotted line) for A-C substitutions. Both methods yield an overall error rate of 4% in base calling.