Figure 4

Mutated start site. Visualization of a gene identified with a mutation in the start site in Artemis genome browser. RNA-Seq based coverage graph clearly shows expression upstream of the annotated start site of the gene |gi|153091350. When the ORF encompassing the gene |gi|153091350 and the expressed intergenic region (EIR, yellow) upstream of the gene, was used for conducting BLASTX searches, we identified a full length match suggesting that the actual start site is at 'leucine' (probably mutated), instead of 'valine' (based on existing annotation).