Figure 2From: Statistical characterization of multiple-reaction monitoring mass spectrometry (MRM-MS) assays for quantitative proteomicsA comparison of the various methods for calculating limit of detection (LOD, lower line in a pair) and limit of quantification (LOQ, upper line in a pair). The four methods compared are described in Section 4. The method using blank + low concentration sample is the most reliable, and consistently produces acceptable LOD and LOQ values for most practical purposes. The blank only method is a close second, but can under-estimate the LOD and LOQ. The calibration curve method results in very conservative estimates, while the RSD limit method is inconsistent with some extremely low LOD and LOQ values.Back to article page