Localization of RAD5 using 4-NQO selected replicate 2. The red line and shaded region show the inferred allele frequencies in the pool using MULTI POOL, and the red pluses plot the observed allele frequencies from the sequencing data (scale on left axis). Regions without pluses do not have polymorphisms or mappable reads. The magenta dots show LOD scores computed using tests of allele frequencies calculated using 10kb sliding windows, while the green line shows the LOD scores calculated using MULTI POOL (scale on right axis). The gray box shows the position of RAD5, the verified causal gene in the region.