Figure 3

Structural clustering of PYLIS regions from iORFs in the clusters for known pyrrolysine incorporating genes. The figure shows that there are certain structural groupings which roughly correspond to gene fami-lies. Almost all transposase genes are in the green cluster (26 sequences), but the cluster also contains sequences from the mtmB (5 sequences), mttB (2 sequences) and mtbB (2 sequences) families. This is also a quite diverse cluster in terms of maximal distance between elements. The orange cluster (12 sequences) predominantly contains mttB genes (8 sequences), but also includes the TetR genes (2 sequences), a single transposase1 gene and a single mtmB gene. The purple cluster (9 sequences) is a mix of mttB genes (4 sequences) and mtmB genes (4 sequences), but includes a single spurious transposase gene. The red cluster (12 sequences) is predominantly mtmB (8 sequences), but includes also two mttB genes and two mtbB genes. The blue cluster (15 sequences) mostly contains mtbB genes (13 sequences) and includes a distant sub-cluster having a mttB gene and a transposase1 gene.