Figure 2From: Bacterial cell identification in differential interference contrast microscopy imagesDIC shear direction estimation procedure. DIC shear direction estimation from image of spherical bead of diameter 1 [μ m]. An estimated orientation a μ  = 43.52 [deg] (red line) was calculated with a rotated linear structuring element defined by l = 20 [pixels] and n = 180. Scale bar corresponds to 1 [μ m].Back to article page