Figure 8

Levey-Jennings chart for cross-run quality control. Levey-Jennings charts can help visualize changes in a quality control metric over time, across runs, and across material lots. This Levey-Jennings chart shows expected ranges for EC50s for standards run for the ENV2 analyte. The transform script calculated these EC50s using the Ruminex package and 5pl curve fits, so they are displayed on the EC50 - 5pl Rumi tab of this interface.

This example shows differences in expected EC50 ranges for two groups of runs, each of which is associated with a different lot of experimental materials. The example also shows a trend over time in EC50, which might suggest decay in experimental conditions or materials. Such decay would require further investigation. It might require exclusion of results from certain days or material lots, replacement of old reagents, investigation of material supply consistency, or other such measures. Early identification of such a trend can allow a lab to take corrective measures before a quality metric falls outside of acceptable bounds and assay runs must be rejected. Outliers visible in this plot are flagged for review, as shown in Figure 9. Charts can be exported to PDF using the PDF icon.