Figure 9

Active and inactivated quality control flags. Metrics used for cross-run quality control (4pl and 5pl EC50s, AUC and HighMFI for standard or quality control titrations) are flagged for review when they fall outside the maximum expected range. As visualized in Figure 8, the maximum expected range around the mean for a metric is +/−3 times the standard of deviation of the relevant “guide set” (benchmark group of runs). Flagged metrics are highlighted in red, plus listed in a separate column, which facilitates review. Users can inactivate quality control flags for metrics that have been reviewed and deemed acceptable. Once a metric for a run has been inactivated, its red highlighting is removed and its listing is crossed out, as shown here for the 5pl EC50 for the run at the top of the list.