Figure 7

Standard histology stains (top, middle) and hyperspectral imaging (bottom) applied to adjacent sections of 1mm breast cancer biopsies. Hyperspectral imaging is quantitative and our methods allow the pathologist to separate several chemical constituents consistently across tissue samples. Adjacent sections of 1mm biopsy cores are shown stained using hematoxylin and eosin (a), cytokeratin for epithelium (b), and visualized using our methods (c). The SpecVis visualization shows epithelial cells in green, collagen in red, and necrosis in blue. Separating epithelium and stromal cells in H&E stained tissue can be difficult (a), however these differences are visible using a cytokeratin stain (b) for epithelium. Necrosis is difficult to identify using chemical staining (a and b), but can be clearly segmented using mid-IR spectroscopic imaging combined with our processing methods (c, blue).