Table 5 Predicted changes in interaction energy for AA changes in the broadly-neutralizing anti-HIV antibody 4E10

VH

K 14 R

−2.54

VH

K 20 T

−1.975

VH

T 29 S

−3.071

VH

S 36 T

−2.343

VH

I 39 L

−3.495

VH

Q 48 R

−2.846

VH

I 56 V

−1.889

VH

I 59 L

−3.449

VH

G 63 T

−2.573

VH

A 65 T

−1.801

VH

Q 69 P

−1.691

VH

K 70 R

−2.226

VH

V 76 I

−1.678

VH

K 82 R

−2.143

VH

M 89 L

−2.158

VH

S 92 N

−2.549

VH

S 96 P

−2.451

VH

I 109 T

−0.923

VH

I 111 G

−1.026

VH

F 111a W

−2.648

VH

V 111c W

−3.922

VH

V 112d L

−2.517

VH

I 112c G

−3.328

VH

I 112b K

−45.027

VH

D 113 G

−45.886

VH

D 116 A

5.989

VH

V 117 H

−0.445

VH

M 123 L

−1.908

V-KAPPA

L 11 Q

−2.11

V-KAPPA

S 30 G

−3.713

V-KAPPA

S 36 N

0.412

V-KAPPA

S 37 N

−2.128

V-KAPPA

Y 38 K

−55.314

V-KAPPA

K 45 R

−2.614

V-KAPPA

A 68 P

−2.604

V-KAPPA

T 69 S

−2.681

V-KAPPA

I 71 V

−2.177

V-KAPPA

P 72 A

−2.41

V-KAPPA

S 109 Q

−2.969

V-KAPPA

P 115 L

−4.398

V-KAPPA

W 116 S

−5.325

V-KAPPA

I 126 V

−2.531