Figure 6
From: Routine performance and errors of 454 HLA exon sequencing in diagnostics
Experimental setup. One library was created and used for all six runs, for run 1-3 beads emPCR and enrichment was performed together, hence they use the same bead pool. Beads for run 4 were prepared separately. Runs 1-4 use automatic enrichment on a liquid handling robot together with the REM e. Beads for run 5 and 6 were generated independently and enrichment was performed manually.