Figure 1
From: 3D time series analysis of cell shape using Laplacian approaches
The workflow of the proposed approach includes five major steps: cell segmentation, topology fixing, spherical parameterization, shape representation, and shape comparison. Quantitative analyses of cell shape firstly require segmentation of individual cells. The topology of the segmentation might need fixing to create a simply connected 3D object, a requirement for the subsequent step of spherical parameterization. Based on the topology fixed binary volume and the spherical parameterization result, shape representation techniques such as spherical harmonics are employed to describe cell shapes. After that, measurements of local and global dynamic cell shape changes can be conducted.