Figure 4
Whole-movie analysis of population growth rates and doubling times. Whole-movie analysis of population growth rates and doubling times. (A) Mean cell densities over 66 fields of view (blue line) and according standard deviation (light blue patch) per m m2 covering the full experiment time range. Red circles indicate the manually determined number of cells in 4 randomly chosen fields of view at 12 hours, 2 days 4.5 days and 5.5 days as described in Table 1. (B) Increase of cells plotted in log-scale. A non-exponential growth phase could be identified until day 2. Between day 2 and day 5 the number of cells increased exponentially. (C) Population doubling times (blue line) per time point and cell cycle times of ∼1600 manually tracked cells with mean lifetimes from 0.5 to 5 days (gray circles). The doubling time decreased until day 2, where it roughly stabilized around 9 to 11 hours. The cell cycle times coincided with the derived doubling times, indicating a correct automatic derivation.