Figure 2

A differential equation model to quantify temporal phenotypes. (a) Cell populations were permitted to enter and leave states with four different transition rates: k_IM, k_MI, k_MP and k_D. Representative images of cellular states are shown within the nodes. (b) Transition rates were allowed to vary over time, as modelled by parametric sigmoid functions. In the negative control spot (left), the interphase-to-mitosis transition rate k_IM inflected at τ_IM = 54.5 h, as the capacity of the spot to support a growing number of cells became limiting. The death transition rate k_D remained constant and null. In the siCOPB1 spot (right), the death transition rate k_D inflected at τ_D = 29.4 h, indicating the time of cell death. (c) Cell population time courses (light solid lines) and fitted data (dotted lines) of the spots described in panel b.