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Table 2 Estimated parameter values and confidence intervals

From: Mechanistic analysis of multi-omics datasets to generate kinetic parameters for constraint-based metabolic models

  Parameter Value 95% Confidence Units
PTS kcat 20.7 ±9.1 mmol/mg protein/hr
PGI kcat 40.2 ±9.5 mmol/mg protein/hr
  Keq 1.23 ±0.29 Dimensionless
PFK kcat 26 ±35 mmol/mg protein/mM/hr
ALDO kcat 3.965 ±0.010 mmol/mg protein/mM/hr
  Keq 0.18 ±0.17 mM
  Kfdp 0.0074 ±0.0036 mM
TPI kcat 10000 ±14000 mmol/mg protein/hr
  Keq 0.11400 ±0.00031 Dimensionless
GAPD kcat 10000 ±4100 mmol/mg protein/mM2/hr
  Keq 1.21 ±0.14 Dimensionless
PGM kcat 9995 ±40 mmol/mg protein/hr
  Keq 0.53570 ±0.00063 Dimensionless
PGK kcat 54.3 ±2.9 mmol/mg protein/hr
  Keq 5512.1 ±1.2 Dimensionless
ENO kcat 2 ±45 mmol/mg protein/mM/hr
  Keq 1.4 ±4.1 Dimensionless
PYK kcat 40 ±49 mmol/mg protein/hr
PDH Kcat 10.4 ±4.6 mmol/mg protein/hr
  Kpyr 0.000020 ±0.000029 mM4
PPC kcat 2.15 ±1.80 mmol/mg protein/hr
  Kfdp 2.5 ±6.5 mM
  Kpep 0.10 ±0.16 mM
G6PDH kcat 859.6 ±1.1 mmol/mg protein/mM2/hr
GND kcat 18.5 ±10.8 mmol*mM/mg protein/hr
  K6pg 0.021 ±0.012 mM
RPI kcat 549.46 ±0.69 mmol/mg protein/mM/hr
  Keq 1.40000 ±0.00019 Dimensionless
RPE kcat 10000 ±13000 mmol/mg protein/mM/hr
  Keq 0.4900 ±0.0044 Dimensionless
TKT1 kcat 10000 ±7800 mmol/mg protein/mM2/hr
  Keq 1.99 ±0.012 Dimensionless
TKT2 kcat 10000 ±5800 mmol/mg protein/mM2/hr
  Keq 3.500 ±0.013 Dimensionless
TALA kcat 10000 ±2300 mmol/mg protein/mM2/hr
  Keq 0.3675 ±0.0021 Dimensionless
  1. Binding coefficient parameters have units of mM, except for K pyr in PDH, which has units of mM4. Equilibrium constants (K eq ) are dimensionless, except for ALDO, which has units of mM. Values for kcat have units such that fluxes have units of mmol/gDW/h. Global upper and lower bounds for binding and enzyme activity were 104 and 10-6, respectively. Bounds on equilibrium constants were 0.35K eq o and 2.85K eq o, where K eq o is the measured equilibrium constant.