Table 2 Sources of false positive junction detection
Type of error | False positives | Qualitative filtering strategy | Removed by qualitative filtering1 | Removed by quantitative filtering2 |
|---|---|---|---|---|
False alignment to minor form | 36.4% | Remove novel minor forms | 36.4% | 30.7% |
Incorrect strand | 31.6% | Inconsistency with gene model | 31.6% | 28.8% |
Shifted on same strand | 13.8% | None | 0% | 12.2% |
Paralog joining | 8.5% | Inconsistency with gene model | 8.5% | 7.7% |
Repeat sequence induced | 7.7% | Exon-intron sequence similarity | 7.7% | 6.5% |
Unidentified error | 2% | None | 0% | 0% |
Total defined errors: | 100% | Total removed errors: | 84.2% | 75.9% |