Skip to main content

Table 1 Comparison of analysis of treatment groups by both the automated motion analysis technique and observer quantification

From: A novel application of motion analysis for detecting stress responses in embryos at different stages of development

a) Danio rerio
Developmental stage Treatment 5 salinity Control   
  Automated Manual Automated Manual   
19 hpf Control ns ns     
  1.5% EtOH ns ns ns ns   
21.5 hpf Control ns ns     
1.5% EtOH * * ns ns   
33 hpf Control ns ns     
1.5% EtOH * ns *** **   
b) Xenopus laevis
Developmental stage Treatment 20 salinity     
   Automated Manual     
St. 24 Control *** *     
St. 32 Control *** ns     
St. 34 Control *** ns     
c) Radix balthica
Dev. stage Treatment Control 5 salinity 10 salinity
   Automated Manual Automated Manual Automated Manual
E3 5 salinity * ns     
  10 salinity ** *** ns ns   
  15 salinity ** *** ** * ** *
E4 5 salinity * *     
  10 salinity ** *** ** ns   
  15 salinity ** *** ** *** * *
E6 5 salinity ** ***     
  10 salinity ** *** ns ns   
  15 salinity ** *** ** ns ** ns
E9 5 salinity ns ns     
  10 salinity ** * * ns   
  15 salinity ** * ** ns * ns
E11 5 salinity * ns     
  10 salinity ** * * ns   
  15 salinity ** *** ** * ** *
  1. ANOSIM of Bray-Curtis similarity matrices calculated from the results of Discrete Fourier Transform on frame-to-frame motion parameters. Manual quantification of movement patterns: number of tail flicks for Danio rerio (19 hpf - F17 = 1.23, P = 0.321; 21.5 hpf - F17 = 4.70, P = 0.026; 33 hpf - F17 = 11.83, P = 0.001) and Xenopus laevis (St. 24 - F11 = 8.98, P = 0.013; St. 32 - F11 = 3.38, P = 0.096; St. 34 - F11 = 3.16, P = 0.106) and number of embryo rotations for Radix balthica (E3 - F23 = 9.36, P = ≤ 0.001; E4 - F23 = 26.33, P = ≤ 0.001; E6 - F23 = 58.25, P = ≤ 0.001; E9 - F23 = 8.40, P = 0.001; E11 - F23 = 7.88, P = 0.001); ANOVA between treatment groups to tests for differences in the manual quantification of embryonic movement for (a) Danio rerio, (b) Xenopus laevis and (c) Radix balthica. *** - p ≤ 0.001, ** - p ≤ 0.01, * - p ≤ 0.05, ns - not significant.