A novel application of motion analysis for detecting stress responses in embryos at different stages of development

Table 1 Comparison of analysis of treatment groups by both the automated motion analysis technique and observer quantification

ANOSIM of Bray-Curtis similarity matrices calculated from the results of Discrete Fourier Transform on frame-to-frame motion parameters. Manual quantification of movement patterns: number of tail flicks for Danio rerio (19 hpf - F₁₇ = 1.23, P = 0.321; 21.5 hpf - F₁₇ = 4.70, P = 0.026; 33 hpf - F₁₇ = 11.83, P = 0.001) and Xenopus laevis (St. 24 - F₁₁ = 8.98, P = 0.013; St. 32 - F₁₁ = 3.38, P = 0.096; St. 34 - F₁₁ = 3.16, P = 0.106) and number of embryo rotations for Radix balthica (E3 - F₂₃ = 9.36, P = ≤ 0.001; E4 - F₂₃ = 26.33, P = ≤ 0.001; E6 - F₂₃ = 58.25, P = ≤ 0.001; E9 - F₂₃ = 8.40, P = 0.001; E11 - F₂₃ = 7.88, P = 0.001); ANOVA between treatment groups to tests for differences in the manual quantification of embryonic movement for (a) Danio rerio, (b) Xenopus laevis and (c) Radix balthica. *** - p ≤ 0.001, ** - p ≤ 0.01, * - p ≤ 0.05, ns - not significant.

ISSN: 1471-2105