Figure 5From: Blind spots of quantitative RNA-seq: the limits for assessing abundance, differential expression, and isoform switchingComparison of reported expression counts with the number of reads sequenced. The biases are dominated by the way the reads with multiple alignments are taken into account. Multiple counting leads to overestimation while ignoring multi-reads leads to underestimation. The most accurate results are achieved by the posterior estimates of RSEM.Back to article page