Figure 2
From: ZebIAT, an image analysis tool for registering zebrafish embryos and quantifying cancer metastasis
Segmentation procedure. Top left: Image from the green fluorescence channel. Top right: Image filtered with LoG. Middle left: Result from LoG edge detection. Middle right: Result from Otsu's threshold. Bottom left: Segmented image after all steps, including morphological closing. Bottom right: Outline of the fish. For illustration purposes, the images are presented with inverted grayscale values.