Figure 7
From: Assessing the impact of human genome annotation choice on RNA-seq expression estimates
Workflow of RNA-seq data analysis pipeline. The five dashed boxes correspond to five steps in the RNA-seq data analysis pipeline. We applied two alignment tools and two quantification tools to estimate gene/isoform expression with normalization methods of count, TPM, or FPKM. The fold-change method and the edgeR tool are used to infer differentially expressed genes. At each analysis step, we assess the variations resulting from genome annotation choice.